Advanced Cell Diagnostics

Provider of the most advanced
RNA in situ hybridization technology
Simultaneous flow cytometric analysis of IFN-γ and CD4 mRNA and protein expression kinetics in human peripheral blood mononuclear cells during activation., Van Hoof D, Lomas W, Hanley MB, Park E., Cytometry A., 2014 August 13 --- Effect of local application of an antibody against brain-derived neurotrophic factor on neuroma formation after transection of the inferior alveolar nerve in the rat., Valverde Guevara YM, Yoshikawa H, Saito I, Maeda T, Seo K., Neuroreport, 2014 July 22 --- Temporal control over the initiation of cell motility by a regulator of G-protein signaling., Hartwig J, Tarbashevich K, Seggewiß J, Stehling M, Bandemer J, Grimaldi C, Paksa A, Groß-Thebing T, Meyen D, Raz E., Proceedings of the National Academy of Sciences of the United States of America, 2014 July 21 --- A Novel Chromogenic In Situ Hybridization Assay for FGF23 mRNA in Phosphaturic Mesenchymal Tumors., Carter JM, Caron BL, Dogan A, Folpe AL., American Journal of Surgical Pathology, 2014 July 14 --- Oncofetal Long Noncoding RNA PVT1 promotes proliferation and stem cell-like property of hepatocellular carcinoma cells by stabilizing NOP2, Wang F, Yuan JH, Wang SB, Yang F, Yuan SX, Ye C, Yang N, Zhou WP, Li WL, Li W, Sun SH, Hepatology, 2014 July 12 --- X-inactivation normalizes O-GlcNAc Transferase levels and generates an O-GlcNAc-depleted Barr body, Olivier-van Stichelen, S, J. A. Hanover, Frontiers in Genetics, 2014 July 11 --- Early colonizing Escherichia coli elicits remodeling of rat colonic epithelium shifting toward a new homeostatic state., Tomas J, Reygner J, Mayeur C, Ducroc R, Bouet S, Bridonneau C, Cavin JB, Thomas M, Langella P, Cherbuy C., ISME J., 2014 July 11 --- Endogenous intrahepatic IFNs and association with IFN-free HCV treatment outcome., Meissner EG, Wu D, Osinusi A, Bon D, Virtaneva K, Sturdevant D, Porcella S, Wang H, Herrmann E, McHutchison J, Suffredini AF, Polis M, Hewitt S, Prokunina-Olsson L, Masur H, Fauci AS, Kottilil S., J Clin Invest, 2014 July 01 --- Human Conchal Cartilage and Temporal Fascia: An Evidence-based Roadmap from Rhinoplasty to an In Vivo Study and Beyond., Cimpean AM, Crăiniceanu Z, Mihailovici D, Bratu T, Raica M., In Vivo. , 2014 July 01 --- PROX1 Expression in Gastric Cancer: From Hypothesis to Evidence., Taban O, Cimpean AM, Raica M, Olariu S., Anticancer Res., 2014 July 01 ---


Delivers Quantitative Molecular Detection and Morphological Context (MD & MC) in a Single Assay

RNAscope® Technology is a cutting-edge multiplex nucleic acid in situ hybridization technology, based on ACD’s unique, patented probe design strategy which enables simultaneous signal amplification and background noise suppression.  RNAscope Technology represents one of the most significant advances in ISH technology in over 40 years.

What is the value of Quantitative Molecular Detection and Morphological Context (MD & MC) in a single assay? What makes RNAscope unique?

  • Molecular Detection: RNAscope detects single RNA molecules and provides quantitative analysis of gene expression at the single-cell resolution.
  • Morphological Context: RNAscope localizes RNA molecules providing spatial and cell-specific expression while preserving tissue architecture.
  • Quantitative: RNAscope quantifies RNA molecules per cell, giving accurate expression level at the single cell resolution across heterogeneous cell populations. 
  • Universal:  RNAscope's patented probe design can be applied to virtually ANY gene in ANY genome in ANY tissue.
  • Scalable: The assay is robust and easy to implement in basic, translational and clinical research labs. Availability of Manual Assays, Automated Assays and Assay Services provide ultimate flexibility.

RNAscope enables in situ detection of RNA biomarkers—filling a long standing gap in in situ molecular analysis.

RNAscope can complement IHC when antibodies are unavailable or have insufficient sensitivity or uncertain specificity. RNAscope is ideal for detecting RNAs for secreted proteins and non-coding RNA—a limitation of IHC.

Downstream of microarrays and NGS (e.g., RNA-seq), RNAscope is an ideal platform for translating new scientific advances into clinical applications due to the speed of RNAscope assay development and its superior sensitivity and specificity.

Other RNA biomarker analysis methods such as RT-PCR can provide quantitative, sensitive and specific measurements of RNA transcripts. However, the resulting quantitative gene expression information is without morphological and cellular context and only represents a “group average” of an admixture of heterogeneous populations of cells. RNAscope’s ability to provide gene expression at the single cell resolution coupled with morphological context is well-suited for RNA analysis in tissue diagnostics applications.

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