RNAscope® Multiplex Reagent Kits
ACD's patented RNAscope technology and proprietary probe design methodology ensure universal assay conditions for every gene, enabling simultaneous multiplex detection of virtually any target RNA gene combination in situ.
Each RNAscope Multiplex Reagent Kit includes all of the necessary assay reagents for chromogenic in situ hybridization (except target probes), including: Pretreatment Kit, Detection Kit and Wash Buffer.
Each assay provides the same exceptional sensitivity as our single-plex assays, allowing single-molecule detection of multiple RNA targets simultaneously, and are ideal for co-localization studies of any expressed gene set in nearly any tissue type.
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RNAscope 2-Plex Chromogenic Assays Feature:
- Simultaneous single-molecule RNA detection of 2 target genes
- Visualize the relative localization of both target genes
- Exceptional sensitivity and specificity for both target genes
- Easy IHC-like workflow with ready-to-use reagents
- Applicable to FFPE or Fresh Frozen tissues
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RNAscope Multiplex Fluorescent Assays Feature:
- Simultaneous single-molecule RNA detection of up to 4 target genes
- Visualize the relative localization of all target genes
- Exceptional sensitivity and specificity for each target gene
- Simple, robust, 6-hour manual assay
- 2-plex or 3-plex off-the-shelf kits (DAPI, RITC, FITC, Cy5)
- 4-plex custom kits (DAPI, RITC, FITC, Cy5, infra-red)
- Mix-and-match probe-fluorophore combinations
- Fully optimized protocol for Fresh Frozen tissue
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The HybEZ Hybridization System is recommended for routine performance of all single-plex and multiplex RNAscope FFPE Assays. All of our Manual RNAscope FFPE assays are specifically developed and validated using the HybEZ System. Optimal performance of Manual RNAscope Assays for in situ hybridization can only be guaranteed when using the HybEZ.
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Catalog # |
RNAscope Fluorescent Multiplex Products |
320850 |
RNAscope Fluorescent Multiplex Reagent Kit
for Fresh Frozen Tissues |
320842 |
RNAscope Fluorescent Pretreatment Kit
for Fresh Frozen Tissues |
320851 |
RNAscope Fluorescent Multiplex Reagent Kit
for Fresh Frozen Tissues |
310091 |
RNAscope Wash Buffer |
300031 |
1-plex Target Probes |
300037 |
2-plex Target Probes |
300038 |
3-plex Target Probes |
300039 |
4-plex Target Probes |
320861 |
3-plex Positive Control Probe - Human |
320871 |
3-plex Negative Control Probe |
320881 |
3-plex Positive Control Probe - Mouse |
320891 |
3-plex Positive Control Probe - Rat |
Catalog # |
RNAscope Duplex Products |
320700 |
RNAscope 2-Plex FFPE Reagent Kit |
320701 |
RNAscope 2-Plex FFPE Detection Kit |
| 310020 |
Pretreatment Kit |
| 310091 |
Wash Buffer |
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HybEZ Hybridization System |
300037 |
RNAscope 2-Plex Target Probe, email your gene pair to order@acdbio.com |
320741 |
RNAscope 2-Plex Housekeeping Gene Positive Control Probe
(PPIB-red-pos + PolR2a-green-pos) |
320751 |
RNAscope 2-Plex Negative Control Probe
(DapB-red-neg + DapB-green-neg) |
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- Each unit provides enough RNAscope in situ hybridization reagents for staining 20 slides.
- Each RNAscope FFPE Reagent kit includes all of the necessary assay reagents and wash buffers to perform manual in situ hybridization using the HybEZ oven, except Target Probe.
- RNAscope 2-Plex FFPE Target Probes must be ordered separately, and a probe set-up fee is required for all 2-Plex Target Probe orders.
- The set-up fee for 2-Plex Target Probe will be waived FOR RE-ORDERS ONLY.
- Target Probe Search is for single-plex ONLY.
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- ApoE4 (red) + LGR5 (green)in fresh frozen mouse cerebellum
- PanCK (red) + uPA (green) in breast cancer tissue
- EPCAM1 (red) + EGFR (green) in breast cancer tissue
- Ig-Kappa (red) + Ig-Lambda (green) in lymphoma tissue
For more information, please read Product Support.
