PRODUCTS

RNAscope® 2-Plex Chromogenic FFPE Reagent Kits

 

Ig Kappa Lambda in FFPE tonsil tissue

HybEZ

For manual use with the

HybEZ Hybridization System

2-Plex Target Probe Sets can be ordered for virtually any pair of genes!

 

Our patented RNAscope technology and proprietary probe design methodology ensure universal assay conditions for every gene, enabling simultaneous multiplex detection of virtually any target RNA gene combination in situ.

 

Each RNAscope 2-Plex FFPE Reagent Kit includes all of the necessary assay reagents for chromogenic in situ hybridization (except target probes), including: Pretreatment Kit, Detection Kit and Wash Buffer.

 

 

RNAscope 2-Plex Assays Feature:

  • Simultaneous single-molecule RNA detection of 2 target genes
  • Visualize the relative localization of both target genes
  • Exceptional sensitivity and specificity for both target genes
  • Easy IHC-like workflow with ready-to-use reagents
 

 

 

 

The HybEZ Hybridization System is recommended for routine performance of all single-plex and multiplex RNAscope FFPE Assays. All of our Manual RNAscope FFPE assays are specifically developed and validated using the HybEZ System.  Optimal performance of Manual RNAscope Assays for in situ hybridization can only be guaranteed when using the HybEZ.

 

320700

RNAscope 2-Plex FFPE Reagent Kit

320701

RNAscope 2-Plex FFPE Detection Kit

310020 Pretreatment Kit
310091 Wash Buffer

 

HybEZ Hybridization System

300037

RNAscope 2-Plex Target Probe, email your gene pair to order@acdbio.com

320741

RNAscope 2-Plex Housekeeping Gene Positive Control Probe

(PPIB-red-pos + PolR2a-green-pos)

320751

RNAscope 2-Plex Negative Control Probe

(DapB-red-neg + DapB-green-neg)

 

  • Each unit provides enough RNAscope in situ hybridization reagents for staining 20 slides.
  • Each RNAscope FFPE Reagent kit includes all of the necessary assay reagents and wash buffers to perform manual in situ hybridization using the HybEZ oven, except Target Probe.
  • RNAscope 2-Plex FFPE Target Probes must be ordered separately, and a probe set-up fee is required for all 2-Plex Target Probe orders.
  • The set-up fee for 2-Plex Target Probe will be waived FOR RE-ORDERS ONLY.
  • Target Probe Search is for single-plex ONLY.

 

 

ApoE4 + LGR5 stained with RNAscope 2-Plex FFPE Assay PanCK + uPA stained with RNAscope 2-Plex FFPE Assay PCAM1 + EGFR staining with RNAscope 2-Plex FFPE Assay Ig-kappa + Ig-lambda stained with RNAscope 2-Plex FFPE Assay

 

  1. ApoE4 (red) + LGR5 (green)in fresh frozen mouse cerebellum
  2. PanCK (red) + uPA (green) in breast cancer tissue
  3. EPCAM1 (red) + EGFR (green) in breast cancer tissue
  4. Ig-Kappa (red) + Ig-Lambda (green) in lymphoma tissue

 

 

 

For more information, please read Product Support.