Advanced Cell Diagnostics

Provider of the most advanced
RNA in situ hybridization technology
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AUTOMATED RNASCOPE® ASSAYS

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RNASCOPE® REAGENT KITS — ONE ASSAY FOR VIRTUALLY ANY GENE!

For labs that require high throughput and automated RNA in situ hybridization (ISH) analysis, ACD offers RNAscope®VS 2.0 and RNAscope®LS 2.0 Chromogenic Reagent Kits as a fully automated walk-away solution on the DISCOVERY ULTRA and DISCOVERY XT automated tissue staining systems by Ventana Medical Systems, Inc and the Leica BOND RX System by Leica Biosystems. The systems automated the Hybridize and Amplify steps of the RNAscope Workflow. 

High-Throughput Discovery & Validation of Tissue-Based Biomarkers

RNAscope®VS 2.0 and RNAscope®LS 2.0 assays can be easily developed for essentially the entire human transciptome. If you have a novel gene or sequence, we can design an assay that is guaranteed to work in < 3 weeks. With full automation you can discover and validate your biomarkers faster than ever before. And the end result is an assay with the performance of a clinical-grade biomarker assay, optimized for FFPE tissues and performed on the same research instrument as for IHC.

WHICH AUTOMATED RNASCOPE® ASSAY IS RIGHT FOR ME?

Use the table below to learn more about which RNA in situ hybridization (ISH) product is right for your research needs.

Assays & Features RNAscope® Ventana Assay Chromogenic Reagent Kit - BROWN RNAscope® Ventana Assay Chromogenic Reagent Kit - RED RNAscope® Leica Assay Chromogenic Reagent Kit - BROWN
Automated System Ventana DISCOVERY ULTRA
Ventana DISCOVERY XT
Ventana DISCOVERY ULTRA
Ventana DISCOVERY XT
Leica BOND RX System
Assay Type Chromogenic Chromogenic Chromogenic
Dye Used Diaminobenzidine (DAB) Fast Red Diaminobenzidine (DAB)
Multiplexing Singleplex Singleplex Singleplex
Key Benefit Robust, sensitive, permanent stain
Most widely used RNAscope assay
Provides bright color stains with high contrast to background Robust, sensitive, permanent stain
Most widely used RNAscope assay
Ideal  for High throughput 
Routine applications
Studies of tissues with endogenous color background such as melanin in skin, liver, or lung from smokers High throughput
Routine applications
Novel Gene or Unkown Expression +++++ +++++ +++++
Archival Specimens +++++ ++ +++++
Microscope Imaging System Standard bright field Standard bright field Standard bright field
Sample Type FFPE FFPE FFPE
Quantitation Method Visual inspection
RNAscope® SpotStudio™ Software automated quantitation
Visual inspection
RNAscope® SpotStudio™ Software automated quantitation
Visual inspection
RNAscope® SpotStudio™ Software automated quantitation
# of tissue sections per kit 60  (20 mm x 20 mm) 60  (20 mm x 20 mm) 60  (20 mm x 20 mm)

 

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