Advanced Cell Diagnostics

Provider of the most
sensitive and specific RNA
in situ hybridization technology
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RNAscope® Assays

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RNAscope® Reagent Kits — One assay for virtually ANY gene!

Our easy RNA in situ hybridization assay format uses common reagents and protocols to provide universal assay conditions for different target genes. All RNA-ISH kits contain reagents in a convenient ready-to-use (RTU) format. RNAscope® Reagent Kits are available in singleplex and multiplex chromogenic and fluorescent formats. We also offer automated assays for Ventana Systems (VS)  and Leica Systems (LS), giving you the flexibility you need for your clinical research study design and size.

Which RNAscope® Assay is right for me?

Use the table below to learn more about which RNA in situ hybridization product is right for your research needs.


RNAscope® Assays & Features

RNAscope® 2.0 HD Assay-BROWN 

RNAscope® 2.0 HD Assay-RED

RNAscope® 2-plex Assay

RNAscope® Multiplex Fluorescent Assay

Assay Type Chromogenic Chromogenic Chromogenic Fluorescent
Dye Used Diaminobenzidine (DAB)  Fast Red Fast Red 
HRP Green

FITC, Cy3, Cy5, Cy7

RNAscope® Probes

Channel Designation

Channel 1

(C1 Probes)

Channel 1

(C1 Probes)

Channels 1 & 2

(C1 & C2 Probes)

Channels 1-4

(C1,C2,C3,C4 Probes)

Multiplexing Singleplex Singleplex Singleplex, Duplex Single to Fourplex
Key Benefit

Robust, sensitive, permanent stain
Most widely used RNAscope assay

Provides bright color stains with high contrast to background

Utilizes 2 different staining enzymes therefore avoiding any cross talk between the two stains

Utilizes up to 4 different spectral channels providing high flexibility

Ideal  for First time users
Routine applications

Studies of tissues with endogenous color background such as melanin in skin, liver, or lung from smokers

Studies interrogating 2 RNA biomarkers 

Co-localization studies of up to 4 RNA biomarkers
Experimental applications requiring flexiblity

Novel Gene or Unkown Expression +++++ +++++ +++ +++
Archival Specimens +++++ ++ +++ ++
Microscope Imaging System Standard bright field

Standard bright field
Multispectral fluorescent imaging

Standard bright field Multispectral fluorescent imaging

Sample Type



Fresh Frozen Tissue


Fresh Frozen Tissue


Fresh Frozen Tissue


Fresh Frozen Tissue
Quantitation Method

Visual inspection
RNAscope® SpotStudio™ Software automated quantitation

Visual inspection
RNAscope® SpotStudio™ Software automated quantitation

Visual inspection Visual inspection

Formalin-fixed, paraffin-embedded (FFPE), Tissue-Microarray (TMA), Peripheral Blood Mononuclear Cells (PBMCs), Circulating Tumor Cells (CTC)

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