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RNAscope Multiplex

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INTRODUCTION

The RNAscope® Multiplex Assay is based on ACD’s patented signal amplification and background suppression technology. The assay uses a novel and proprietary method of in situ hybridization (ISH) to visualize single RNA molecules per cell in fresh frozen tissue samples mounted on slides. RNAscope® Assays do not require the RNA-free environment used for traditional ISH. This assay provides the same exceptional sensitivity as our single-plex assays, allowing single-molecule detection of multiple RNA targets simultaneously and is ideal for co-localization studies of any expressed gene set in nearly any tissue type.

Each RNAscope® Multiplex Fluorescent Reagent Kit provides enough reagents to stain ~20 tissue sections approximately 20 mm x 20 mm large. Larger tissue sections will result in fewer tests. Each kit contains three sub-kits: a Pretreatment Kit, a Detection Kit, and a Wash Buffer Kit.

KEY FEATURES AND BENEFITS

  1. Simultaneous detection of up to four RNA species
  2. Single-molecule detection
  3. Ready-to-use reagents
  4. Compatible with fresh tissue

APPLICATIONS

Gene Expression Analysis, Neuroscience, Target Identification and Validation, Toxicology, Tumor Heterogeneity

ASSAY COMPONENTS

320850 - RNAscope Fluorescent Multiplex Reagent Kit for FF

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DETAILS

320851 - RNAscope Fluorescent Multiplex Detection Kit for FF

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DETAILS

320970 - RNAscope® PBMC Sample Prep Kit

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DETAILS

310020 - RNAscope Pretreatment Kit

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DETAILS

310091 - RNAscope Wash Buffer

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DETAILS
hybridization assay
RNA ISH:HPRT1, POLR2A, PPIB and PGK mRNA, human breast cancer FFPE tissue,RNA In Situ Hybridization:Beta-Actin, HPRT1, RPLP0, and PPIB expression in HeLa cell with RNAscope® Multiplex Fluorescent Assay,In Situ Hybridization:IL-8 and beta-Actin mRNA expression in stimulated cultures cells with RNAscope® Multiplex Fluorescent Assay,RNA Hybridization:Npy and Fexf2 mRNA expression in mouse brain fresh frozen tissue with RNAscope® Multiplex Fluorescent Assay,RNA ISH:SNCA and TH63 mRNA expression in mouse cerebellum with RNAscope® Multiplex Fluorescent Assay,RNA In Situ Hybridization:HCV and 18s mRNA expression in HCV infected cells with RNAscope® Multiplex Fluorescent Assay,In Situ Hybridization:Fezf2, NPY, and APOE mRNA expression in mouse brain fresh frozen tissue with RNAscope® Multiplex Fluorescent Assay,RNA Hybridization:panCK, uPA and Pai-1 mRNA expression in FFPE breast cancer tissue with RNAscope® Multiplex Fluorescent Assay,RNA ISH:OMP and OLFR73 mRNA,, fresh-frozen olfactory bulb
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